The Mouse Cyclin-dependent Kinase 8 (CDK8) ELISA Kit offered by Assay Genie is a highly accurate and reliable tool for the detection of CDK8 levels in mouse serum, plasma, and cell culture supernatants. This kit utilizes advanced technology to ensure precise and consistent results, making it ideal for a wide range of research applications.CDK8 is a key regulator of cell cycle progression and gene expression, playing a vital role in various cellular processes. Dysregulation of CDK8 has been implicated in numerous diseases, including cancer and metabolic disorders, highlighting its importance as a potential therapeutic target.
With its high sensitivity and specificity, the Mouse CDK8 ELISA Kit from Assay Genie is a valuable tool for researchers looking to study the role of CDK8 in different disease conditions and develop novel treatment strategies. Trust Assay Genie for accurate and reliable results in your research endeavors.
Product Name:
Mouse Cyclin Dependent Kinase 8 (CDK8) ELISA Kit
Product Code:
MODL00246
Size:
96 Assays
Target:
CDK8
Synonyms:
K35, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
