Mouse Cyclin-dependent kinase 5 (Cdk5) ELISA Kit

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SKU:
MOEB0567
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P49615
ELISA Type:
Sandwich
Synonyms:
CDK5, Cyclin-dependent kinase 5, TPKII catalytic subunit, Tau protein kinase II catalytic subunit, Cell division protein kinase 5, Serine, threonine-protein kinase PSSALRE
Reactivity:
Mouse
Frequently bought together:

Description

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Product Name:Mouse Cyclin-dependent kinase 5 (Cdk5) ELISA Kit
Product Code:MOEB0567
Alias:Cyclin-dependent kinase 5, CR6 protein kinase, CRK6, Cell division protein kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit, Cdk5, Cdkn5, Crk6, 2.7.11.22
Uniprot:P49615
Reactivity:Mouse
Range:Please contact us for more information
Detection Method:Sandwich
Size:96 Assay
Storage:Please see kit components below for exact storage details
Note:For research use only
UniProt Protein Function:CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Kinase, protein; Protein kinase, CMGC; EC 2.7.11.1; CMGC group; CDK family; CDK5 subfamily; CDK/CDK5 subfamily

Cellular Component: cyclin-dependent protein kinase 5 activator complex; cell; dendrite; postsynaptic density; cytosol; postsynaptic membrane; growth cone; cytoskeleton; cell projection; cell soma; membrane; axon; lamellipodium; cytoplasm; nucleolus; plasma membrane; synapse; intracellular; neuromuscular junction; nucleus; cell junction; filopodium

Molecular Function:acetylcholine receptor activator activity; ephrin receptor binding; p53 binding; nucleotide binding; protein kinase binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; ErbB-2 class receptor binding; protein binding; ErbB-3 class receptor binding; cyclin-dependent protein kinase activity; tau-protein kinase activity; transferase activity, transferring phosphorus-containing groups; cytoskeletal protein binding; kinase activity; ATP binding

Biological Process: Schwann cell development; negative regulation of synaptic plasticity; regulation of cell cycle; cerebellar cortex formation; rhythmic process; regulation of synaptic plasticity; motor axon guidance; sensory perception of pain; receptor clustering; protein amino acid phosphorylation; regulation of cell migration; corpus callosum development; neuron differentiation; cerebellar cortex development; receptor catabolic process; oligodendrocyte differentiation; cerebellum development; neurite development; central nervous system neuron development; synaptic transmission, glutamatergic; nervous system development; skeletal muscle development; dendrite morphogenesis; regulation of postsynaptic membrane potential; cell division; synaptic vesicle endocytosis; negative regulation of protein ubiquitination; cerebral cortex development; negative regulation of transcription, DNA-dependent; synaptic transmission, dopaminergic; regulated secretory pathway; negative regulation of protein export from nucleus; exocytosis; positive regulation of protein binding; apoptosis; cell-matrix adhesion; protein amino acid autophosphorylation; neuron migration; nucleocytoplasmic transport; negative regulation of axon extension; negative regulation of cell cycle; intracellular protein transport; synaptogenesis; behavioral response to cocaine; positive regulation of neuron apoptosis; forebrain development; response to wounding; visual learning; cortical actin cytoskeleton organization and biogenesis; associative learning; layer formation in the cerebral cortex; serine phosphorylation of STAT3 protein; negative regulation of proteolysis; cell migration; hippocampus development; peptidyl-threonine phosphorylation; cell cycle; response to cocaine; positive regulation of calcium ion-dependent exocytosis; telencephalon development; peptidyl-serine phosphorylation; neuron apoptosis; axonogenesis; positive regulation of protein kinase activity; regulation of excitatory postsynaptic membrane potential; phosphorylation; neurite morphogenesis

UniProt Code:P49615
NCBI GenInfo Identifier:6680908
NCBI Gene ID:12568
NCBI Accession:NP_031694.1
UniProt Related Accession:P49615
Molecular Weight:33,288 Da
NCBI Full Name:cyclin-dependent kinase 5
NCBI Synonym Full Names:cyclin-dependent kinase 5
NCBI Official Symbol:Cdk5  
NCBI Official Synonym Symbols:Crk6; AW048668  
NCBI Protein Information:cyclin-dependent-like kinase 5; CR6 protein kinase; TPKII catalytic subunit; cell division protein kinase 5; serine/threonine-protein kinase PSSALRE; tau protein kinase II catalytic subunit
UniProt Protein Name:Cyclin-dependent-like kinase 5
UniProt Synonym Protein Names:CR6 protein kinase; CRK6; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
Protein Family:Cyclin-dependent kinase
UniProt Gene Name:Cdk5  
UniProt Entry Name:CDK5_MOUSE
Component Quantity (96 Assays) Storage
ELISA Microplate (Dismountable) 8×12 strips -20°C
Lyophilized Standard 2 -20°C
Sample Diluent 20ml -20°C
Assay Diluent A 10mL -20°C
Assay Diluent B 10mL -20°C
Detection Reagent A 120µL -20°C
Detection Reagent B 120µL -20°C
Wash Buffer 30mL 4°C
Substrate 10mL 4°C
Stop Solution 10mL 4°C
Plate Sealer 5 -

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.

Step
1. Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C.
2. Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform.
3. Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4. Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C.
5. Repeat the wash process for five times as conducted in step 3.
6. Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction.
7. Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8. Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9. After experiment, store all reagents according to the specified storage temperature respectively until their expiry.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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