The Mouse CXCL16 (Chemokine C-X-C Motif Ligand 16) ELISA Kit is specifically designed for the accurate detection of CXCL16 levels in mouse serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures reliable and reproducible results, making it perfect for a variety of research applications.CXCL16 is a chemokine protein that plays a vital role in immune response and inflammation by recruiting leukocytes to sites of infection or injury. It is also involved in a range of diseases, including cancer, autoimmune disorders, and cardiovascular diseases, making it an important biomarker for studying these conditions and potential therapeutic development.
This ELISA kit provides researchers with a valuable tool for studying the role of CXCL16 in various physiological and pathological processes, allowing for accurate quantification and analysis in mouse models. Get accurate and reliable results with the Mouse CXCL16 ELISA Kit.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCL16. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CXCL16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CXCL16, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CXCL16. You can calculate the concentration of Mouse CXCL16 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-104
97-109
89-104
Average (%)
95
102
96
1:4
Range (%)
94-107
82-95
94-106
Average (%)
101
87
101
1:8
Range (%)
95-108
89-102
93-106
Average (%)
101
94
99
1:16
Range (%)
100-114
86-99
97-112
Average (%)
107
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.4
214.8
792.3
93.2
197.8
735.6
Standard deviation
6.5
11.0
39.6
6.4
11.7
33.8
C V (%)
6.81
5.12
5.0
6.87
5.92
4.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CXCL16 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CXCL16 in samples. No significant cross-reactivity or interference between Mouse CXCL16 and analogues was observed.
