Mouse CTSB Recombinant Protein (RPPB1514)
- SKU:
- RPPB1514
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- CTSB
- Synonyms:
- Cathepsin B
- Cathepsin B1
- CTSB
- Source:
- Sf9 Insect cells
- Uniprot:
- P10605
Description
| Product Name: | Mouse CTSB Recombinant Protein |
| Product Code: | RPPB1514 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | CTSB |
| Synonyms: | Cathepsin B, Cathepsin B1, CTSB. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | CTSB protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | HDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGS CWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPP CTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RFLEHHHHHH |
| Biological Activity: | Specific activity is > 2,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C. |
Cathepsin B preproprotein (CTSB) is a papain-family cysteine protease which is normally located in lysosomes. CTSB is able to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of some human and rodent tumors progression and arthritis.
CTSB Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 330 amino acids (18-339a.a.) and having a molecular mass of 36.4kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).CTSB is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; EC 3.4.22.1; Protease; Autophagy Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; mitochondrion; lysosome; extracellular region; caveola; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; nucleolus; sarcolemma; external side of plasma membrane Molecular Function:peptidase activity; collagen binding; proteoglycan binding; protein self-association; hydrolase activity; cysteine-type endopeptidase activity; endopeptidase activity; protein complex binding; kininogen binding; peptide binding; cysteine-type peptidase activity Biological Process: collagen catabolic process; proteolysis involved in cellular protein catabolic process; regulation of catalytic activity; decidualization; proteolysis |
| NCBI Summary: | This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P10605 |
| NCBI GenInfo Identifier: | 115712 |
| NCBI Gene ID: | 13030 |
| NCBI Accession: | P10605.2 |
| UniProt Secondary Accession: | P10605,Q3UDW7, |
| UniProt Related Accession: | P10605 |
| Molecular Weight: | 37,280 Da |
| NCBI Full Name: | Cathepsin B |
| NCBI Synonym Full Names: | cathepsin B |
| NCBI Official Symbol: | Ctsb |
| NCBI Official Synonym Symbols: | CB |
| NCBI Protein Information: | cathepsin B; cathepsin B1 |
| UniProt Protein Name: | Cathepsin B |
| UniProt Synonym Protein Names: | Cathepsin B1Cleaved into the following 2 chains:Cathepsin B light chain; Cathepsin B heavy chain |
| Protein Family: | Cathepsin |
| UniProt Gene Name: | Ctsb |
| UniProt Entry Name: | CATB_MOUSE |
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