Mouse cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
The Mouse cTnT (TNNT2) Troponin T Type 2 Cardiac ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of troponin T levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying cardiac function and cardiovascular diseases in mouse models.Troponin T is a key regulatory protein in cardiac muscle contraction and a well-established biomarker for myocardial injury.
Elevated troponin T levels in the blood are indicative of cardiac damage, making it a valuable marker for diagnosing and monitoring conditions such as heart attacks and heart failure.The Mouse cTnT (TNNT2) Troponin T Type 2 Cardiac ELISA Kit offers researchers a precise and efficient method for detecting and quantifying troponin T in mouse samples, facilitating studies on cardiac function, disease mechanisms, and potential therapeutic interventions in mouse models of cardiovascular diseases.
Product Name:
Mouse cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
SKU:
MOES01677
Target:
Mouse cTnT/TNNT2 (Troponin T Type 2, Cardiac)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse cTnT/TNNT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse cTnT/TNNT2. You can calculate the concentration of Mouse cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
85-97
88-101
Average (%)
102
92
93
1:4
Range (%)
91-107
87-97
86-100
Average (%)
98
92
92
1:8
Range (%)
93-108
82-96
80-95
Average (%)
100
88
87
1:16
Range (%)
87-101
79-92
84-99
Average (%)
93
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.6
114.86
427.77
47.7
116.26
469.6
Standard deviation
2.45
5.96
22.07
2.63
6.21
16.2
C V (%)
5.37
5.19
5.16
5.51
5.34
3.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse cTnT/TNNT2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Mouse cTnT/TNNT2 and analogues was observed.
