Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Proteins CTLA4 / CD152 Immune Checkpoint ProteinsImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Mouse CTLA4/CD152 Recombinant Protein (RPES1637)
Product Code:
RPES1637
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
Cd152,Ctla-4,Ly-56
Accession:
NP_033973.2
Sequence:
Met 1-Phe 162
Fusion tag:
C-His
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized recombinant mouse CTLA4-His at 10 µg/ml (100 ?l/well) can mouse CD80-Fc with a linear range of 0.78-62.5 ng/ml.3. Immobilized recombinant mouse CTLA4-His at 10 µg/ml (100 ?l/well) can human B7-1-Fc with a linear range of 0.78-125 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) was expressed, with a polyhistidine tag at the C-terminus.
Purity:
> 98 % as determined by SDS-PAGE
Mol Mass:
15.3 kDa
AP Mol Mass:
25-30 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
