The Mouse CT (Calcitonin) ELISA Kit is specifically designed for the precise measurement of calcitonin levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and selectivity, guaranteeing accurate and consistent results, making it an indispensable tool for a variety of research studies.Calcitonin is a key hormone that regulates calcium and phosphate metabolism, as well as bone remodeling. Its levels are crucial for maintaining bone health and have been linked to conditions such as osteoporosis and hypercalcemia.
By accurately measuring calcitonin levels, researchers can better understand the role of this hormone in various physiological and pathological processes, leading to potential therapeutic advancements in bone-related disorders.Don't miss out on the opportunity to explore the intricate world of calcitonin with the Mouse CT (Calcitonin) ELISA Kit and unlock valuable insights into bone health and related conditions.
Product Name:
Mouse CT (Calcitonin) ELISA Kit
SKU:
MOES00793
Target:
Mouse CT (Calcitonin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CT. You can calculate the concentration of Mouse CT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
93-105
91-104
Average (%)
93
99
96
1:4
Range (%)
94-109
84-94
97-114
Average (%)
102
89
105
1:8
Range (%)
95-110
84-97
93-109
Average (%)
101
91
100
1:16
Range (%)
97-111
83-99
95-112
Average (%)
105
90
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
85-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.4
91.0
395.8
55.8
99.9
401.8
Standard deviation
2.9
4.6
16.2
3.4
4.3
21.3
C V (%)
5.43
5.05
4.09
6.09
4.3
5.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CT in samples. No significant cross-reactivity or interference between Mouse CT and analogues was observed.
