The Mouse CRT (Calreticulin) ELISA Kit is specifically designed for the precise measurement of calreticulin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Calreticulin is a key protein that plays a vital role in various cellular processes, including calcium homeostasis, chaperone activity, and immunogenicity regulation.
Dysregulation of calreticulin has been linked to a range of diseases, such as cancer, autoimmune disorders, and cardiovascular conditions, highlighting its significance as a biomarker for these ailments and potential therapeutic development.Get your Mouse CRT (Calreticulin) ELISA Kit today from Assay Genie and take your research to the next level.
Product Name:
Mouse CRT (Calreticulin) ELISA Kit
SKU:
MOES00798
Target:
Mouse CRT (Calreticulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CRT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CRT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CRT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CRT. You can calculate the concentration of Mouse CRT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-114
88-99
86-98
Average (%)
104
94
91
1:4
Range (%)
90-104
84-95
81-96
Average (%)
97
89
87
1:8
Range (%)
90-102
84-96
86-101
Average (%)
96
91
93
1:16
Range (%)
95-107
81-92
83-96
Average (%)
100
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
97
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.07
4.41
0.49
0.98
4.71
Standard deviation
0.04
0.06
0.23
0.02
0.04
0.24
C V (%)
7.69
5.61
5.22
4.08
4.08
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CRT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CRT in samples. No significant cross-reactivity or interference between Mouse CRT and analogues was observed.
