The Mouse Collagen Type VI Alpha 1 (COL6A1) ELISA Kit is a valuable tool for researchers looking to accurately measure levels of COL6A1 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.COL6A1 is a key component of collagen type VI, a protein involved in maintaining the structural integrity of various tissues in the body. Dysregulation of COL6A1 has been linked to conditions such as muscular dystrophy, fibrosis, and certain skin disorders.
By reliably measuring COL6A1 levels, researchers can gain important insights into disease mechanisms and potential therapeutic targets.Overall, the Mouse Collagen Type VI Alpha 1 (COL6A1) ELISA Kit is a valuable tool for studying the role of collagen type VI in various physiological and pathological processes, providing researchers with the necessary data to advance their understanding of these complex mechanisms.
Product Name:
Mouse Collagen Type VI Alpha 1 (COL6a1) ELISA Kit
Product Code:
MODL00293
Size:
96 Assays
Target:
COL6a1
Synonyms:
COL6-A1, OPLL, Collagen Alpha-1(VI)chain
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
