The Mouse Collagen Type I Alpha 2 (COL1A2) ELISA Kit is specifically designed for the accurate measurement of COL1A2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.COL1A2 is a key component of type I collagen, the most abundant collagen in the extracellular matrix of connective tissues. It plays a critical role in maintaining the structural integrity and strength of tissues such as bone, skin, and tendons.
Dysregulation of COL1A2 expression is associated with various pathological conditions, including osteogenesis imperfecta, osteoporosis, and fibrosis, making it an important biomarker for studying these diseases and developing therapeutic interventions.Overall, the Mouse Collagen Type I Alpha 2 ELISA Kit provides researchers with a valuable tool for investigating the role of COL1A2 in physiological and pathological processes, offering reliable and accurate results for advanced scientific studies.
Product Name:
Mouse Collagen Type I Alpha 2 (COL1a2) ELISA Kit
Product Code:
MODL00288
Size:
96 Assays
Target:
COL1a2
Synonyms:
COL1-A2, OI4, Collagen Alpha-2(I)chain, osteogenesis imperfecta type IV, Collagen Of Skin,Tendon And bone,Alpha-2 Chain
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
