The Mouse COL3 (Collagen Type III) ELISA Kit is specifically designed for the precise measurement of collagen type III levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and consistent results, making it a valuable tool for various research purposes.Collagen type III is a key component of the extracellular matrix, contributing to tissue integrity and function. Changes in collagen type III levels have been linked to various diseases, including fibrosis, cardiovascular disorders, and connective tissue disorders.
This ELISA kit enables researchers to study the role of collagen type III in disease pathology and potential therapeutic interventions.Overall, the Mouse COL3 (Collagen Type III) ELISA Kit offers researchers a reliable and efficient method for investigating the role of collagen type III in health and disease in mouse models.
Product Name:
Mouse COL3 (Collagen Type III) ELISA Kit
SKU:
MOES00880
Target:
Mouse COL3 (Collagen Type III)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse COL3. You can calculate the concentration of Mouse COL3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
96-111
99-116
Average (%)
100
102
106
1:4
Range (%)
92-105
80-92
87-101
Average (%)
97
86
94
1:8
Range (%)
86-99
83-97
84-95
Average (%)
93
89
89
1:16
Range (%)
87-100
85-98
83-99
Average (%)
94
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
93-109
101
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.7
150.12
451.77
46.59
157.32
451.48
Standard deviation
3.13
7.64
22.72
2.8
8.32
17.11
C V (%)
6.56
5.09
5.03
6.01
5.29
3.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse COL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL3 in samples. No significant cross-reactivity or interference between Mouse COL3 and analogues was observed.
