The Mouse COL3 (Collagen Type III) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of collagen type III levels in mouse serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it ideal for various research applications.Collagen type III is a key component of the extracellular matrix, important for maintaining tissue structure and function. Changes in collagen type III levels are associated with conditions such as fibrosis, cardiovascular diseases, and connective tissue disorders.
Studying collagen type III levels can provide valuable insights into the pathophysiology of these diseases and aid in the development of effective therapeutic interventions.The Mouse COL3 (Collagen Type III) CLIA Kit is a powerful tool for researchers studying collagen biology and its role in disease progression. With its high sensitivity and specificity, this kit offers a reliable method for quantifying collagen type III levels in mouse samples, helping advance our understanding of tissue remodeling and fibrotic processes.
Product Name:
Mouse COL3 (Collagen Type III) CLIA Kit
SKU:
MOES00188
Target:
Mouse COL3 (Collagen Type III)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse COL3. You can calculate the concentration of Mouse COL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
103-115
86-99
Average (%)
109
109
91
1:4
Range (%)
90-102
89-100
103-117
Average (%)
97
94
109
1:8
Range (%)
93-106
96-107
101-117
Average (%)
99
101
108
1:16
Range (%)
101-114
86-100
100-114
Average (%)
108
93
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
103
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
19.7
44.04
192.78
18.72
48.14
197.55
Standard deviation
1.8
4.79
16.31
2.15
3.68
12.5
C V (%)
9.14
10.88
8.46
11.49
7.64
6.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse COL3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL3 in samples. No significant cross-reactivity or interference between Mouse COL3 and analogues was observed.
