The Mouse Col2 (Collagen Type II) ELISA Kit is specifically designed for the quantitative detection of collagen type II levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing precise and consistent results for a variety of research purposes.Collagen type II is a key component of cartilage and plays a crucial role in maintaining joint health and function. Abnormalities in collagen type II levels are associated with conditions such as osteoarthritis and other joint-related disorders.
Therefore, this ELISA kit serves as a valuable tool for investigating these conditions and exploring potential therapeutic interventions.Overall, the Mouse Col2 (Collagen Type II) ELISA Kit is an essential resource for researchers focused on understanding cartilage biology, joint diseases, and therapeutic interventions targeting collagen type II. Its accuracy and reliability make it an indispensable tool in the field of musculoskeletal research.
Product Name:
Mouse COL2 (Collagen Type 2) ELISA Kit
SKU:
MOES00930
Target:
Mouse COL2 (Collagen Type 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse COL2. You can calculate the concentration of Mouse COL2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
91-105
93-108
Average (%)
93
98
100
1:4
Range (%)
97-112
87-98
90-107
Average (%)
103
93
97
1:8
Range (%)
97-113
87-99
96-114
Average (%)
104
93
104
1:16
Range (%)
94-106
88-98
91-106
Average (%)
100
93
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
90
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183
574.6
1589.6
190.6
543.7
1507.1
Standard deviation
9.5
34.5
85.8
12.6
29.9
69.3
C V (%)
5.19
6.0
5.4
6.61
5.5
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse COL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL2 in samples. No significant cross-reactivity or interference between Mouse COL2 and analogues was observed.
