The Mouse Col1 (Collagen Type I) ELISA Kit is specifically designed for the precise measurement of Collagen Type I levels in mouse serum, plasma, and tissue culture samples. With its high sensitivity and specificity, this kit delivers accurate and reliable results, making it a valuable tool for researchers in various fields.Collagen Type I is a fundamental component of the extracellular matrix, playing a crucial role in tissue structure, strength, and integrity. Its dysregulation is associated with various diseases, including fibrosis, arthritis, and skin disorders.
Therefore, this ELISA kit provides researchers with a valuable tool to study Collagen Type I levels and its implications in disease development and progression.Overall, the Mouse Col1 (Collagen Type I) ELISA Kit offers researchers a reliable and efficient method to quantitatively measure Collagen Type I levels in mouse samples, leading to a better understanding of its role in health and disease.
Product Name:
Mouse COL1 (Collagen Type I) ELISA Kit
SKU:
MOES00888
Target:
Mouse COL1 (Collagen Type I)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse COL1. You can calculate the concentration of Mouse COL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
92-105
91-103
Average (%)
96
97
98
1:4
Range (%)
85-98
82-97
88-100
Average (%)
92
89
93
1:8
Range (%)
90-104
87-99
83-95
Average (%)
95
92
90
1:16
Range (%)
85-99
82-96
82-97
Average (%)
92
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
2.49
9.3
1.12
2.27
10.16
Standard deviation
0.05
0.13
0.28
0.06
0.12
0.42
C V (%)
4.9
5.22
3.01
5.36
5.29
4.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse COL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL1 in samples. No significant cross-reactivity or interference between Mouse COL1 and analogues was observed.
