The Mouse Col1 (Collagen Type I) CLIA Kit is a highly accurate and reliable assay designed for the detection of collagen type I levels in mouse samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Collagen type I is a key component of the extracellular matrix and plays a vital role in tissue structure, function, and repair. Abnormal collagen type I levels have been associated with various diseases, including fibrosis, arthritis, and other connective tissue disorders.
Therefore, this kit serves as a valuable tool for investigating collagen type I dynamics in disease progression and potential therapeutic interventions.With its user-friendly protocol and high-performance characteristics, the Mouse Col1 (Collagen Type I) CLIA Kit is an essential resource for researchers studying the complex role of collagen type I in health and disease. Visit the website for more information on this innovative assay kit.
Product Name:
Mouse COL1 (Collagen Type I) CLIA Kit
SKU:
MOES00193
Target:
Mouse COL1 (Collagen Type I)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse COL1. You can calculate the concentration of Mouse COL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
91-107
89-104
Average (%)
91
97
95
1:4
Range (%)
87-102
96-112
87-102
Average (%)
94
102
93
1:8
Range (%)
97-109
95-109
102-117
Average (%)
102
101
109
1:16
Range (%)
100-117
88-100
89-103
Average (%)
107
93
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
100
EDTA plasma (n=5)
101-117
107
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.03
387.12
1685.04
202.64
357.47
1817.14
Standard deviation
17.81
37.32
107.17
24.92
40.47
137.19
C V (%)
8.48
9.64
6.36
12.3
11.32
7.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse COL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL1 in samples. No significant cross-reactivity or interference between Mouse COL1 and analogues was observed.
