Mouse Coagulation Factor XIII B Polypeptide (F13B) ELISA Kit
The Mouse Coagulation Factor XIII B Polypeptide (F13B) ELISA Kit is specifically designed for the accurate detection of F13B levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Coagulation Factor XIII B Polypeptide is an important component of the coagulation cascade, playing a key role in the final stages of blood clot formation.
Abnormal levels of F13B have been implicated in a range of clotting disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.With its easy-to-use format and comprehensive instructions, the Mouse F13B ELISA Kit is an essential tool for researchers studying coagulation disorders and related diseases in mouse models. Order yours today to advance your research and discoveries in this important field.
Product Name:
Mouse Coagulation Factor XIII B Polypeptide (F13B) ELISA Kit
Product Code:
MODL00438
Size:
96 Assays
Target:
F13B
Synonyms:
F13-B, FXIIIB, Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
