The Mouse CFB (Complement Factor B) ELISA Kit is specifically designed for the precise measurement of complement factor B levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it perfect for various research purposes.Complement factor B is a key component in the complement system, playing a vital role in immune response and inflammation.
Dysregulation of complement factor B has been linked to various diseases, including autoimmune disorders and inflammatory conditions, making it a valuable biomarker for studying these diseases and exploring potential treatments.Overall, the Mouse CFB ELISA Kit from Assay Genie provides researchers with a reliable and efficient tool for investigating the role of complement factor B in biological processes and disease pathogenesis.
Product Name:
Mouse CFB (Complement Factor B) ELISA Kit
SKU:
MOES00897
Target:
Mouse CFB (Complement Factor B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CFB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CFB. You can calculate the concentration of Mouse CFB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
96-110
97-114
Average (%)
104
101
105
1:4
Range (%)
89-101
80-91
81-94
Average (%)
94
86
87
1:8
Range (%)
88-99
81-92
88-102
Average (%)
94
86
95
1:16
Range (%)
94-107
84-95
84-98
Average (%)
99
89
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
101
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.59
12.3
35.95
4.22
11.63
33.21
Standard deviation
0.24
0.58
1.51
0.29
0.54
1.71
C V (%)
5.23
4.72
4.2
6.87
4.64
5.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CFB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CFB in samples. No significant cross-reactivity or interference between Mouse CFB and analogues was observed.
