The Mouse CEA (Carcinoembryonic Antigen) ELISA Kit is a powerful tool for the quantification of CEA levels in mouse serum, plasma, and tissue lysates. With its exceptional accuracy and precision, this kit provides researchers with dependable and consistent results, enabling the study of CEA expression in various biological samples. CEA is a glycoprotein that is commonly used as a tumor marker for various cancers, including colorectal, lung, breast, and pancreatic cancer. Monitoring CEA levels can aid in cancer diagnosis, prognosis, and treatment response assessment, making this ELISA kit essential for cancer research and clinical applications.
By utilizing the Mouse CEA ELISA Kit, researchers can gain valuable insights into the role of CEA in cancer development and progression, as well as explore potential therapeutic interventions targeting this biomarker. Upgrade your research capabilities with this high-quality ELISA kit from Assay Genie.
Product Name:
Mouse CEA (Carcinoembryonic Antign) ELISA Kit
SKU:
MOES00805
Target:
Mouse CEA (Carcinoembryonic Antign)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CEA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CEA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CEA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CEA. You can calculate the concentration of Mouse CEA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
87-100
92-105
Average (%)
101
94
100
1:4
Range (%)
90-104
87-99
84-99
Average (%)
98
93
91
1:8
Range (%)
87-101
81-92
87-100
Average (%)
93
86
94
1:16
Range (%)
88-103
87-100
85-97
Average (%)
96
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.22
359.17
1607.45
214.32
370.68
1753.47
Standard deviation
11.34
21.12
48.54
12.49
18.35
91.18
C V (%)
5.37
5.88
3.02
5.83
4.95
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CEA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CEA in samples. No significant cross-reactivity or interference between Mouse CEA and analogues was observed.
