CD99 antigen-like protein 2, also known as MIC2-like protein 1, CD99L2 and MIC2L1, is a single-pass type I membrane protein which belongs to the CD99 family. CD99L2 is expressed in brain, heart, lung, liver, spleen, kidney, stomach, small intestine, skeletal muscle, ovary, thymus, testis and uterus. Lower expression of CD99L2 is seen in thymus. It is also expressed in E18 uterus and placenta. CD99 and CD99L2 were required for leukocyte extravasation in the cremaster after stimulation with tumor necrosis factor-alpha, where the need for PECAM-1 is known to be bypassed. CD99 and CD99L2 act independently of PECAM-1 in leukocyte extravasation and cooperate in an independent way to help neutrophils overcome the endothelial basement membrane. CD99L2 may function as a homophilic adhesion molecule. It functions in leukocyte-endothelial cell interactions during leukocyte extravasation, and in particular, at the diapedesis step. CD99L2 does not seem to be involved in docking of leukocytes to the vessel wall or in lymphocyte diapedesis.
Product Name:
Mouse CD99L2 Recombinant Protein (RPES1520)
Product Code:
RPES1520
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AW548191,Mic2l1,Xap89
Accession:
NP_612182.1
Sequence:
Met 1-Ala 164
Fusion tag:
C-His
Activity:
Measured by its ability to bind biotinylated recombinant mouse CD99L2 in functional ELISA.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse CD99L2 (NP_612182.1) extracellular domain (Met 1-Ala 164) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 98 % as determined by SDS-PAGE
Mol Mass:
16.3 kDa
AP Mol Mass:
30-40 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
