The Mouse CD90-2 Monoclonal Antibody (PE-Cy5 Conjugated, 30H12) is a specialized tool for researchers interested in studying CD90-2, a cell surface protein known for its role in cell adhesion and signaling. This antibody is derived from hybridoma technology and conjugated with PE-Cy5 for fluorescence microscopy and flow cytometry applications. It specifically targets the CD90-2 molecule, allowing for precise localization and quantification in various cell populations.CD90-2, also referred to as Thy1.2 antigen, is a well-established marker for identifying and isolating specific cell types, particularly in the context of immunology and stem cell research.
Its versatile applications range from characterizing immune cell subsets to investigating stem cell differentiation pathways. By utilizing this antibody, researchers can gain valuable insights into the functions of CD90-2 in different biological processes and disease models, paving the way for novel therapeutic strategies and clinical interventions.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Gene ID:
21838
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.