T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Product Name:
Mouse CD8a/Lyt2 Recombinant Protein (RPES2830)
Product Code:
RPES2830
Size:
20µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
BB154331,Ly-2,Ly-35,Ly-B,Lyt-2
Accession:
NP_001074579.1
Sequence:
Met 1-Tyr 196
Fusion tag:
C-His
Activity:
1. Measured by its ability to bind biotinylated recombinant human B2M in a functional ELISA.2. Measured by its ability to bind biotinylated recombinant human FCGRT+B2M in a functional ELISA.3. Measured by its ability to bind biotinylated recombinant human LCK in a functional ELISA.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse CD8a (NP_001074579.1) (Met 1-Tyr 196) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
20.3 kDa
AP Mol Mass:
35 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
