Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins.It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.
Product Name:
Mouse CD59a/MAC-IP Recombinant Protein (RPES5081)
Product Code:
RPES5081
Size:
20µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AA987121,Cd59,protectin,RP24-297H17.1
Accession:
O55186
Sequence:
Met 1-Lys 95
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse CD59a (O55186) (Met 1-Lys 95), without the pro peptide, was fused with a C-terminal polyhistidine tag.
Purity:
> 92 % as determined by SDS-PAGE
Mol Mass:
9.7 kDa
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
