The Mouse CD45 Monoclonal Antibody (PE Conjugated, 30-F11, AGEL1658) is a valuable tool for researchers working with mouse samples in immunology and cell biology studies. This antibody specifically targets the CD45 antigen, a key cell surface marker found on all leukocytes, making it an essential tool for identifying and studying various immune cell populations.The PE conjugation of this antibody allows for easy and efficient detection using flow cytometry, making it ideal for analyzing and sorting immune cells based on their CD45 expression levels. Its high specificity and sensitivity ensure reliable results in experiments involving immune cell characterization, immune response studies, and cell signaling pathways.
By utilizing the Mouse CD45 Monoclonal Antibody (PE Conjugated, 30-F11, AGEL1658), researchers can gain valuable insights into the immune system's function, inflammatory responses, and immune cell populations in mouse models. This antibody is a crucial asset for scientists aiming to deepen their understanding of immune-related diseases, develop novel therapies, and advance immunological research.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P06800
Gene ID:
19264
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.