The Mouse CD45.2 Monoclonal Antibody (AF647 Conjugated, 104-2) from Assay Genie (AGEL1534) is a powerful tool for studying immune cell populations in mouse models. This antibody specifically targets CD45.2, a transmembrane protein highly expressed in hematopoietic cells, making it a valuable marker for identifying leukocytes.With its fluorescent AF647 conjugation, this monoclonal antibody offers high sensitivity and specificity for flow cytometry and immunofluorescence applications. It allows for precise localization and quantification of CD45.
2-positive cells, aiding in the characterization of immune cell subtypes and their activation status.Ideal for immunology and cell biology research, the Mouse CD45.2 Monoclonal Antibody (AF647 Conjugated, 104-2) opens new possibilities for investigating immune responses, immune cell development, and immune-related diseases in mouse models. Enhance your studies with this reliable reagent from Assay Genie, a trusted provider of high-quality research tools.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Gene ID:
19264
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
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