The Mouse CD45.1 Monoclonal Antibody APC Conjugated (A20) is a powerful tool for researchers studying immune cell populations in mice. This monoclonal antibody specifically targets the CD45.1 antigen, a common marker found on mouse leukocytes, making it an essential reagent for flow cytometry and immunophenotyping experiments.Derived from the well-known A20 hybridoma cell line, this antibody offers high specificity and sensitivity in detecting CD45.1-positive cells. The APC conjugation provides strong fluorescent signal for robust and accurate cell sorting and analysis. With its excellent performance in flow cytometry applications, this antibody facilitates precise identification and characterization of mouse immune cell subsets.
With its broad utility in immunology research, the Mouse CD45.1 Monoclonal Antibody APC Conjugated (A20) is a valuable asset for investigating immune responses, studying immune cell populations in disease models, and exploring mechanisms of immune regulation in mice. This antibody is a reliable choice for researchers aiming to deepen their understanding of mouse immunology and advance discoveries in the field.
Product Title:
APC Anti-Mouse CD45.1 Antibody [A20]
SKU:
AGEL1950
Size:
25µg, 100µg
Clone No:
A20
Synonyms:
Ly-5;L-CA;T200;CD45;Ptprc;
Host Species:
Mouse
Reactivity:
Mouse
Application:
FCM
Isotype:
Mouse IgG2a, κ
Isotype Control:
APC Mouse IgG2a, κ Isotype Control[C1.18.4]
Form:
Liquid
Conjugation:
APC
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Gene ID:
19264
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.