The Mouse CD4 (Cluster of Differentiation 4) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CD4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a variety of research applications.CD4 is a key glycoprotein present on the surface of T helper cells, playing a crucial role in the immune response by coordinating the activities of other immune cells.
Monitoring CD4 levels is essential for studying immune system function, autoimmune diseases, and infectious diseases like HIV/AIDS.Overall, the Mouse CD4 CLIA Kit is an invaluable tool for researchers interested in studying immune responses and exploring potential therapeutic targets for various diseases.
Product Name:
Mouse CD4 (Cluster Of Differentiation 4) CLIA Kit
SKU:
MOES00176
Target:
Mouse CD4 (Cluster Of Differentiation 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CD4. You can calculate the concentration of Mouse CD4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
93-106
98-115
Average (%)
95
100
106
1:4
Range (%)
95-110
93-105
93-106
Average (%)
101
98
99
1:8
Range (%)
86-100
86-97
88-100
Average (%)
92
91
95
1:16
Range (%)
91-105
98-115
95-109
Average (%)
99
106
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
99-110
105
Cell culture media (n=5)
100-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
257.76
662.78
1780.39
263.65
681.37
1646.77
Standard deviation
27.61
49.05
173.59
27.34
60.3
104.24
C V (%)
10.71
7.4
9.75
10.37
8.85
6.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CD4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CD4 in samples. No significant cross-reactivity or interference between Mouse CD4 and analogues was observed.
