The Mouse CD3 Epsilon Monoclonal Antibody, FITC Conjugated (clone 145-2C11) from Assay Genie (Catalog No. AGEL1315) is a powerful tool for immunological research. This antibody targets the CD3 epsilon subunit, a key component of the T-cell receptor complex that plays a central role in T-cell activation and signaling.By specifically binding to CD3 epsilon, this monoclonal antibody enables precise detection and characterization of T cells in various experimental settings. The FITC conjugation further enhances its utility, allowing for fluorescence-based analyses such as flow cytometry and immunofluorescence microscopy.
Researchers can rely on this antibody to accurately identify and study T cells in both in vitro and in vivo models.With its high specificity and sensitivity, the Mouse CD3 Epsilon Monoclonal Antibody, FITC Conjugated (clone 145-2C11) is a valuable resource for investigating immune responses, understanding T-cell function, and exploring the complexities of adaptive immunity. Whether studying basic immunology, host-pathogen interactions, or immune-mediated diseases, this antibody provides reliable results and facilitates insightful research outcomes.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P22646
Gene ID:
12501
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.