Mouse CD3 (Cluster of differentiation 3) ELISA Kit
The Mouse CD3 (Cluster of Differentiation 3) ELISA Kit is a specialized tool for the quantitative measurement of CD3 levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring robust and consistent results for a variety of research applications.CD3 is a key marker for T cells, playing a critical role in immune responses and autoimmune diseases.
By accurately measuring CD3 levels, researchers can gain valuable insights into immune system function, disease progression, and potential therapeutic targets. This ELISA kit is a valuable tool for studying T cell biology and immunological disorders in mouse models, contributing to advancements in biomedical research.
Product Name:
Mouse CD3 (Cluster of differentiation 3) ELISA Kit
SKU:
MOES00859
Target:
Mouse CD3 (Cluster of differentiation 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CD3. You can calculate the concentration of Mouse CD3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-112
92-107
86-98
Average (%)
102
98
91
1:4
Range (%)
89-100
84-98
88-100
Average (%)
94
91
94
1:8
Range (%)
94-106
82-92
85-99
Average (%)
100
87
92
1:16
Range (%)
90-102
84-96
86-101
Average (%)
96
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.15
2.93
11.86
1.18
3.2
12.54
Standard deviation
0.07
0.16
0.47
0.07
0.13
0.4
C V (%)
6.09
5.46
3.96
5.93
4.06
3.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CD3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CD3 in samples. No significant cross-reactivity or interference between Mouse CD3 and analogues was observed.
