The Mouse CD25 Monoclonal Antibody (PERCP-Cyanine5.5 Conjugated) - PC 61.5.3 (AGEL1311) is a cutting-edge tool designed for researchers studying immune responses and immune cell regulation in mice. This monoclonal antibody specifically targets CD25, a cell surface marker that is crucial for the activation and proliferation of T cells. By conjugating PERCP-Cyanine5.5 to the antibody, it enables precise detection and quantification of CD25-expressing cells in flow cytometry experiments.CD25, also known as interleukin-2 receptor alpha chain, is a key player in the immune system, essential for T cell activation and regulatory T cell function. This monoclonal antibody allows for the identification and analysis of CD25-positive cells, providing valuable insights into immune cell dynamics and function in various experimental settings.
Its high specificity and sensitivity make it a valuable tool for researchers investigating immune responses, T cell signaling pathways, and autoimmune diseases in mouse models.By utilizing the Mouse CD25 Monoclonal Antibody (PERCP-Cyanine5.5 Conjugated) - PC 61.5.3 (AGEL1311), researchers can enhance their understanding of immune cell behavior and functionality, paving the way for advancements in immunology research and the development of novel therapeutic strategies for immune-related disorders in mice.
PerCP/Cyanine5.5 Rat IgG1, κ Isotype Control[HRPN]
Form:
Liquid
Conjugation:
PerCP/Cyanine 5.5
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.