The Mouse CD25 Monoclonal Antibody PE-Cyanine5.5 Conjugated (PC61.5.3) is a vital tool for researchers studying immune responses and cell surface markers in mouse models. This antibody, conjugated with PE-Cyanine5.5 for multi-color flow cytometry applications, specifically targets CD25, also known as the interleukin-2 receptor alpha chain.CD25 is a crucial component of the interleukin-2 receptor complex, playing a pivotal role in T cell activation and regulation. By targeting CD25, researchers can analyze and track activated T cells, regulatory T cells, and other immune cell populations involved in immune responses.
This antibody is validated for flow cytometry, enabling precise detection and quantification of CD25-expressing cells in various tissues and experimental conditions.Studies using the Mouse CD25 Monoclonal Antibody PE-Cyanine5.5 Conjugated can provide insights into immune regulation, tolerance induction, and immune cell function in the context of infections, autoimmune diseases, and cancer. With its high specificity and sensitivity, this antibody is an essential tool for immunologists and researchers investigating the intricate mechanisms of immune system modulation and response regulation in mouse models.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.