The Mouse CD25 Monoclonal Antibody (FITC Conjugated) - PC-61.5.3 (AGEL1305) offered by Assay Genie is a powerful tool for researchers studying immune cells expressing the CD25 antigen, also known as the Interleukin-2 receptor alpha chain. This antibody, derived from mouse hybridoma cells, is conjugated with FITC for easy visualization and detection in flow cytometry and fluorescence microscopy applications.CD25 is a crucial marker for regulatory T cells (Tregs), which play a vital role in maintaining immune tolerance and preventing autoimmune responses. By targeting CD25, researchers can accurately identify and isolate Tregs for in-depth immunological studies.
The high specificity and sensitivity of this monoclonal antibody ensure reliable results and facilitate the investigation of Treg function in various disease models and immune settings.With its exceptional performance and versatility in immunophenotyping experiments, the Mouse CD25 Monoclonal Antibody (FITC Conjugated) - PC-61.5.3 (AGEL1305) is an indispensable tool for advancing research in immunology, autoimmunity, transplantation, and cancer immunotherapy. Get precise insights into Treg biology and functionality with this reliable reagent from Assay Genie.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
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