The Mouse CD25 Monoclonal Antibody (AF647 Conjugated, clone PC61.5.3 - AGEL1313) offered by Assay Genie is a powerful tool for researchers studying immune responses in mouse models. This antibody specifically targets CD25, a cell surface protein also known as the interleukin-2 receptor alpha chain, which plays a crucial role in T cell activation and regulatory T cell function.Developed with high specificity and sensitivity, the AF647-conjugated PC61.5.3 monoclonal antibody allows for the precise detection and visualization of CD25-expressing cells in flow cytometry and immunofluorescence experiments. Its fluorescent conjugation provides enhanced signal intensity, making it an excellent choice for multiplexing with other fluorophores for complex experimental setups.
The use of this antibody can help researchers better understand the dynamics of immune cell activation, tolerance induction, and immune regulation in various experimental models. With its strong performance and reliability, the Mouse CD25 Monoclonal Antibody (AF647 Conjugated, clone PC61.5.3 - AGEL1313) is a valuable asset for studies focusing on immunology, autoimmunity, transplantation, and cancer immunotherapy in mouse research systems.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.