PE/Cyanine7 Anti-Mouse CD162 Antibody [4RA10] (AGEL0851)

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SKU:
AGEL0851
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Applications:
FC
Frequently bought together:

Description

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PE/Cyanine7 Anti-Mouse CD162 Antibody [4RA10]

The Mouse CD162 Monoclonal Antibody (PE/Cyanine7 Conjugated, 4RA10) is a specialized tool for researchers studying CD162, a cell surface molecule with implications in immune regulation and adhesion processes. This antibody, derived from mouse sources, exhibits high specificity and sensitivity for mouse samples, particularly in flow cytometry applications. By binding specifically to CD162, it enables precise detection and characterization of this molecule in diverse cell populations, making it an essential component in immunology and cell biology research.CD162, also known as PSGL-1 (P-selectin glycoprotein ligand-1), is crucial in mediating leukocyte adhesion and migration, playing a pivotal role in inflammatory responses and immune cell recruitment.

Its involvement in leukocyte trafficking makes it a key target for investigations into various immune-related conditions, including inflammatory disorders and vascular diseases. By unraveling the mechanisms and functions of CD162, researchers can gain valuable insights into immune cell behavior and potentially identify new therapeutic strategies for combating inflammatory conditions and enhancing immune responses.

Product Title:PE/Cyanine7 Anti-Mouse CD162 Antibody [4RA10]
SKU:AGEL0851
Size:25µg, 100µg
Clone No:4RA10
Synonyms:P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG1, κ
Isotype Control:PE/Cyanine7 Rat IgG1, κ Isotype Control[HRPN]
Form:Liquid
Conjugation:PE/Cyanine 7
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Swissprot:Q62170
Gene ID:20345
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

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