PE Anti-Mouse CD162 Antibody [4RA10] (AGEL0848)

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SKU:
AGEL0848
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Applications:
FC
Frequently bought together:

Description

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PE Anti-Mouse CD162 Antibody [4RA10]

The Mouse CD162 Monoclonal Antibody (PE Conjugated, 4RA10) is a powerful tool for researchers interested in studying CD162, a key cell surface molecule with implications in immune response modulation. This monoclonal antibody, conjugated with PE for enhanced detection, is specifically designed for use in flow cytometry and immunofluorescence applications.CD162, also known as P-selectin glycoprotein ligand-1 (PSGL-1), is involved in cell adhesion and is crucial for interactions between immune cells and endothelial cells during inflammation and immune response processes. The binding specificity of this antibody to CD162 enables precise detection and analysis in various mouse cell types, making it an ideal choice for investigations in immunology, vascular biology, and inflammatory diseases.

By studying the role of CD162 in immune cell adhesion and migration, researchers can gain insights into the mechanisms underlying inflammatory responses, leukocyte trafficking, and immune cell activation. This antibody provides a valuable tool for exploring the functions of CD162 in various physiological and pathological conditions, advancing our understanding of immune system regulation and potential therapeutic targets for inflammatory disorders.

Product Title:PE Anti-Mouse CD162 Antibody [4RA10]
SKU:AGEL0848
Size:25µg, 100µg
Clone No:4RA10
Synonyms:P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG1, κ
Isotype Control:PE Rat IgG1, κ Isotype Control[HRPN]
Form:Liquid
Conjugation:PE
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Swissprot:Q62170
Gene ID:20345
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

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