Biotin Anti-Mouse CD162 Antibody [4RA10] (AGEL0160)

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SKU:
AGEL0160
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG1
Clone:
4RA10
Conjugation:
Biotin
Applications:
FC
Frequently bought together:

Description

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Biotin Anti-Mouse CD162 Antibody [4RA10]

The Mouse CD162 Monoclonal Antibody (Biotin Conjugated) 4RA10 (AGEL0160) offered by Assay Genie is an essential tool for researchers studying CD162, a cell surface molecule with key implications in immune function. This monoclonal antibody, designed for mouse samples, provides high reactivity and specificity for CD162 protein detection and analysis. With its biotin conjugation, this antibody is suitable for a variety of applications, including immunohistochemistry and flow cytometry.CD162, also known as PSGL-1 (P-selectin glycoprotein ligand-1), is crucial in mediating leukocyte rolling and adhesion during inflammation and immune responses. Its interaction with selectins plays a vital role in immune cell recruitment and trafficking, making it a significant target for investigating inflammatory diseases, such as atherosclerosis and autoimmune disorders.

Understanding the function and regulation of CD162 can provide valuable insights into immune cell behavior, offering opportunities for therapeutic interventions targeting inflammatory pathways.The Mouse CD162 Monoclonal Antibody (Biotin Conjugated) 4RA10 (AGEL0160) from Assay Genie is a reliable tool for researchers aiming to delve into the complexities of immune cell interactions and inflammatory processes mediated by CD162. Its specificity and versatility make it a valuable asset in immunological studies and research focused on elucidating the roles of CD162 in various pathological conditions.

Product Title:Biotin Anti-Mouse CD162 Antibody [4RA10]
SKU:AGEL0160
Size:25µg, 100µg
Clone No:4RA10
Synonyms:P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG1, κ
Isotype Control:Biotin Rat IgG1, κ Isotype Control[HRPN]
Form:Liquid
Conjugation:Biotin
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Swissprot:Q62170
Gene ID:20345
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

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