APC Anti-Mouse CD162 Antibody [4RA10] (AGEL0849)

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SKU:
AGEL0849
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Applications:
FC
Frequently bought together:

Description

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APC Anti-Mouse CD162 Antibody [4RA10]

The Mouse CD162 Monoclonal Antibody (APC Conjugated, 4RA10) is a powerful tool for researchers studying CD162, a cell surface molecule known for its role in immune responses and cell adhesion. This monoclonal antibody, conjugated with APC for enhanced detection, is specifically designed for use in flow cytometry applications to analyze and quantify the expression of CD162 in mouse samples.CD162, also referred to as PSGL-1, is a crucial player in leukocyte trafficking and activation, facilitating interactions between immune cells and endothelial cells during inflammation and immune surveillance. By targeting CD162 with this antibody, researchers can gain insights into the dynamics of immune cell adhesion and migration in various biological contexts, including studies related to inflammation, autoimmune diseases, and infection.

With its high specificity and sensitivity, the Mouse CD162 Monoclonal Antibody (APC Conjugated, 4RA10) provides a reliable tool for investigating the functions of CD162 in immune regulation and inflammatory processes. This antibody is an essential component for flow cytometry-based research projects focused on understanding the complexity of immune cell interactions and exploring potential therapeutic targets for immunological disorders.

Product Title:APC Anti-Mouse CD162 Antibody [4RA10]
SKU:AGEL0849
Size:25µg, 100µg
Clone No:4RA10
Synonyms:P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG1, κ
Isotype Control:APC Rat IgG1, κ Isotype Control[HRPN]
Form:Liquid
Conjugation:APC
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Swissprot:Q62170
Gene ID:20345
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

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