The Mouse CD162 Monoclonal Antibody (4RA10) (AGEL0159) from AssayGenie is an essential tool for researchers studying CD162, a cell surface molecule with significant implications in immune function. This monoclonal antibody, specifically designed for use with mouse samples, is generated using hybridoma technology, ensuring high specificity and sensitivity.CD162, also known as P-selectin glycoprotein ligand-1 (PSGL-1), is involved in immune responses and cell adhesion processes. The Mouse CD162 Monoclonal Antibody (4RA10) (AGEL0159) allows for accurate detection and analysis of CD162 expression in various cell types and tissues, making it an invaluable resource for immunologists and researchers focusing on inflammation, leukocyte trafficking, and vascular biology.
This antibody is optimized for use in techniques such as flow cytometry, immunofluorescence, and immunohistochemistry, providing versatile applications for investigating CD162 expression and function. With its quality validation and reliable performance, the Mouse CD162 Monoclonal Antibody (4RA10) (AGEL0159) is a valuable tool for elucidating the roles of CD162 in immune cell interactions, inflammatory processes, and disease pathogenesis.
Product Title:
Purified Anti-Mouse CD162 Antibody [4RA10]
SKU:
AGEL0159
Size:
25µg, 100µg
Clone No:
4RA10
Synonyms:
P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM;Block
Isotype:
Rat IgG1, κ
Isotype Control:
Purified Rat IgG1, κ Isotype Control[HRPN]
Form:
Liquid
Conjugation:
Unconjugated
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.