The Mouse CD16/32 Monoclonal Antibody (PE/Cyanine5.5 Conjugated, 2.4G2) is a valuable tool for research involving mouse immune cells, particularly macrophages and dendritic cells. This antibody, conjugated with PE/Cyanine5.5 for fluorescent detection, specifically targets CD16 and CD32 antigens, which are crucial markers for identifying and studying these immune cell populations.The CD16/32 antibody is highly specific and has been rigorously validated for flow cytometry applications, enabling precise detection and analysis of mouse immune cells expressing these surface antigens. Its versatility allows for use in various experimental settings and applications, including immunophenotyping, cell sorting, and functional studies.
CD16 and CD32 are key receptors involved in immune responses, such as phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). Understanding the expression and function of these receptors in mouse immune cells is essential for elucidating immune mechanisms in infection, inflammation, and autoimmune diseases. This monoclonal antibody provides researchers with a reliable tool to advance their studies in immunology, infectious diseases, and beyond.
Product Title:
PE/Cyanine5.5 Anti-Mouse CD16/32 Antibody [2.4G2]
SKU:
AGEL0583
Size:
50 Tests, 100 Tests, 200 Tests
Clone No:
2.4G2
Synonyms:
CD16a/b;CD32;CD32A/B;Fc fragment of IgG low affinity IIa/b receptor;Fc fragment of IgG low affinity IIIa/b receptor;Fc fragment of IgG low affinity IIIb receptor;Fc gamma receptor III A/B;FCG2A;FcGR;FCGR2A/BFCGR3;FCGR3A/B;Fc gamma RIIa/b ;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG2b, κ
Isotype Control:
PE/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
PE/Cyanine 5.5
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P08508 P08101
Gene ID:
14130,14131
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.
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