The CD16/32 Monoclonal Antibody (AGEL0595) is a specialized tool designed for researchers studying immune cells, particularly macrophages and natural killer cells expressing CD16 and CD32 surface markers. This antibody, conjugated with Alexa Fluor 488 for fluorescent detection, offers high sensitivity and specificity in detecting these specific cell populations in various biological samples.CD16 and CD32 are crucial receptors involved in antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis, key functions of the immune system in defense against pathogens and cancer cells. The AGEL0595 antibody allows for precise identification and quantification of CD16/32-expressing cells in flow cytometry, immunofluorescence, and other experimental techniques, facilitating detailed analyses of immune cell populations in research settings.
With its superior performance and reliability, the CD16/32 Monoclonal Antibody (AGEL0595) is an essential tool for investigating immune responses, cellular interactions, and therapeutic strategies targeting immune cells expressing CD16 and CD32 markers. Researchers in immunology, oncology, and other fields will benefit from the precision and efficiency this antibody provides in their studies.
CD16a/b;CD32;CD32A/B;Fc fragment of IgG low affinity IIa/b receptor;Fc fragment of IgG low affinity IIIa/b receptor;Fc fragment of IgG low affinity IIIb receptor;Fc gamma receptor III A/B;FCG2A;FcGR;FCGR2A/BFCGR3;FCGR3A/B;Fc gamma RIIa/b ;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG2b, κ
Isotype Control:
Genie Fluor 488 Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
Genie Fluor488
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P08508 P08101
Gene ID:
14130,14131
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.