The Mouse CD16/32 Monoclonal Antibody (AF488 Conjugated, 2.4G2, AGEL0585) is a powerful tool for research involving the CD16/32 antigen, essential for successful immunological probing in mouse models. This antibody, conjugated with AF488 for fluorescence applications, is specifically designed to target the CD16/32 protein, enabling precise detection and analysis of cell populations expressing this marker.CD16/32, also known as Fc-gamma receptors III and II, plays a crucial role in mediating various immune functions, including phagocytosis and antibody-dependent cellular cytotoxicity. Understanding the expression and activity of CD16/32 is vital for unraveling the complexities of immune responses in conditions such as infections, autoimmune diseases, and cancer.
This monoclonal antibody facilitates accurate identification and characterization of CD16/32-expressing cells, offering valuable insights into immune system functionality and pathogenesis.Ideal for use in flow cytometry, immunofluorescence, and other immunological techniques, the Mouse CD16/32 Monoclonal Antibody (AF488 Conjugated, 2.4G2, AGEL0585) is a reliable reagent for researchers aiming to delve deeper into the mechanisms underlying immune responses in mouse models.
CD16a/b;CD32;CD32A/B;Fc fragment of IgG low affinity IIa/b receptor;Fc fragment of IgG low affinity IIIa/b receptor;Fc fragment of IgG low affinity IIIb receptor;Fc gamma receptor III A/B;FCG2A;FcGR;FCGR2A/BFCGR3;FCGR3A/B;Fc gamma RIIa/b ;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG2b, κ
Isotype Control:
Genie Fluor 488 Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
Genie Fluor488
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P08508 P08101
Gene ID:
14130,14131
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.