Mouse CD14 Recombinant Protein (RPPB3008)
- SKU:
- RPPB3008
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- CD14
- Synonyms:
- Monocyte differentiation antigen CD14
- Myeloidcell-specific leucine-rich glycoprotein
- CD14
- Source:
- Sf9 Insect cells
- Uniprot:
- P10810
Description
| Product Name: | Mouse CD14 Recombinant Protein |
| Product Code: | RPPB3008 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | CD14 |
| Synonyms: | Monocyte differentiation antigen CD14, Myeloidcell-specific leucine-rich glycoprotein, CD14. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CD14 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | SPAPPEPCEL DEESCSCNFS DPKPDWSSAF NCLGAADVEL YGGGRSLEYLLKRVDTEADL GQFTDIIKSL SLKRLTVRAA RIPSRILFGA LRVLGISGLQ ELTLENLEVT GTAPPPLLEATGPDLNILNL RNVSWATRDA WLAELQQWLK PGLKVLSIAQ AHSLNFSCEQ VRVFPALSTLDLSDNPELGE RGLISALCPLKFPTLQVLAL RNAGMETPSG VCSALAAARV QLQGLDLSHN SLRDAAGAPS CDWPSQLNSL NLSFTGLKQV PKGLPAKLSVLDLSYNRLDR NPSPDELPQV GNLSLKGNPF LDSESHSEKF NSGVVTAGAP SSQAVALSGT LALLLGDRLF VHHHHHH |
CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.
CD14 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 357 amino acids (16-366a.a.) and having a molecular mass of 38.3kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).CD14 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor Cellular Component: extracellular space; cell surface; membrane; plasma membrane; lipid raft Molecular Function:lipopolysaccharide binding Biological Process: immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; positive regulation of tumor necrosis factor production; inflammatory response; positive regulation of cytokine secretion |
| NCBI Summary: | This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P10810 |
| NCBI GenInfo Identifier: | 115957 |
| NCBI Gene ID: | 12475 |
| NCBI Accession: | P10810.1 |
| UniProt Related Accession: | P10810 |
| Molecular Weight: | 39,204 Da |
| NCBI Full Name: | Monocyte differentiation antigen CD14 |
| NCBI Synonym Full Names: | CD14 antigen |
| NCBI Official Symbol: | Cd14 |
| NCBI Protein Information: | monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein |
| UniProt Protein Name: | Monocyte differentiation antigen CD14 |
| UniProt Synonym Protein Names: | Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14 |
| Protein Family: | Monocyte differentiation antigen |
| UniProt Gene Name: | Cd14 |
| UniProt Entry Name: | CD14_MOUSE |
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