Mouse CD109 (Cluster of Differentiation 109) ELISA Kit
The Mouse CD109 (Cluster of Differentiation 109) ELISA Kit is a powerful tool for the quantitative measurement of CD109 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and precise results for a variety of research applications.CD109 is a cell surface glycoprotein that plays a key role in regulating cell growth, differentiation, and immune response. It has been implicated in a range of biological processes, including tumor progression, inflammation, and tissue regeneration.
The Mouse CD109 ELISA Kit provides researchers with a reliable method to study the function and expression of CD109 in various disease models and therapeutic interventions.Overall, the Mouse CD109 ELISA Kit is an essential tool for investigating the role of CD109 in various biological processes and diseases, ultimately leading to a better understanding of its potential as a therapeutic target. Order yours today from Assay Genie and accelerate your research efforts.
Product Name:
Mouse CD109 (Cluster of Differentiation 109) ELISA Kit
SKU:
MOES00868
Target:
Mouse CD109 (Cluster of Differentiation 109)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD109. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD109 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD109, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CD109. You can calculate the concentration of Mouse CD109 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-106
94-107
99-114
Average (%)
97
100
106
1:4
Range (%)
89-102
84-95
89-104
Average (%)
95
90
95
1:8
Range (%)
86-101
79-94
89-100
Average (%)
92
86
95
1:16
Range (%)
88-98
84-97
89-102
Average (%)
93
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
92-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.53
7.14
1.0
2.75
6.89
Standard deviation
0.06
0.12
0.23
0.07
0.13
0.37
C V (%)
5.5
4.74
3.22
7.0
4.73
5.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CD109 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CD109 in samples. No significant cross-reactivity or interference between Mouse CD109 and analogues was observed.
