Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.
Product Name:
Mouse CCNE1/Cyclin-E1 Recombinant Protein (RPES4014)
Product Code:
RPES4014
Size:
20µg
Species:
Mouse
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
AW538188,CycE1
Accession:
AAH62152.1
Sequence:
Met1-Glu408
Fusion tag:
N-His-GST
Activity:
Measured by its binding ability in a functional ELISA. Immobilized mouse CCNE1 at 10 µg/ml (100 ?l/well) can bind biotinylated human CDK4 , The EC50 of biotinylated human CDK4 is 48.0-114.0 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse CCNE1 (AAH62152.1) (Met1-Glu408) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
