C-C motif chemokine 2 (CCL2) is a member of the C-C or β chemokine family. Mouse CCL2 shares 82% amino acid (aa) identity with rat CCL2 over the entire sequence, and 58%, 56%, 55%, 53% and 53% aa identity with human, equine, porcine, bovine and canine CCL2, respectively. Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source. In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2. Additionally, it promotes Th2 polarization in CD4+ T cells. CCL2-mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma.
Product Name:
Mouse CCL2/MCP Recombinant Protein (RPES5090)
Product Code:
RPES5090
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, MCP-1,Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, Ccl2, Je, Mcp1, Scya2
Accession:
P10148
Sequence:
Gln24-Asn148
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse C-C motif chemokine 2 is produced by our Mammalian expression system and the target gene encoding Gln24-Asn148 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
14.7 kDa
AP Mol Mass:
20-36 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH7.4 .
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
