The Mouse CC17 (Clara Cell) Protein ELISA Kit is a reliable and accurate tool for the quantitative measurement of CC17 levels in mouse serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, providing researchers with trustworthy and reproducible results for various research applications.CC17, also known as Clara Cell Secretory Protein (CCSP), is an important protein primarily expressed in Clara cells of the respiratory epithelium.
It plays a key role in protecting the lungs against oxidative stress and inflammation, making it a valuable biomarker for studying respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), and lung cancer.By utilizing the Mouse CC17 (Clara Cell) Protein ELISA Kit, researchers can gain valuable insights into the role of CC17 in respiratory health and disease, paving the way for potential therapeutic interventions and treatment strategies.
Product Name:
Mouse CC17 (Clara Cell Protein) ELISA Kit
SKU:
MOES00858
Target:
Mouse CC17 (Clara Cell Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CC17. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CC17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CC17, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CC17. You can calculate the concentration of Mouse CC17 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
93-106
93-105
Average (%)
103
99
98
1:4
Range (%)
88-101
81-95
88-103
Average (%)
94
88
95
1:8
Range (%)
88-101
83-96
83-95
Average (%)
95
89
90
1:16
Range (%)
90-107
85-97
86-98
Average (%)
97
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
95
EDTA plasma (n=5)
88-98
93
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.12
3.63
0.5
1.08
3.98
Standard deviation
0.03
0.06
0.14
0.03
0.06
0.21
C V (%)
6
5.36
3.86
6.0
5.56
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CC17 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CC17 in samples. No significant cross-reactivity or interference between Mouse CC17 and analogues was observed.
