The Mouse Casp9 (Caspase-9) ELISA Kit is specifically designed for the accurate measurement of caspase-9 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Caspase-9 is a key enzyme in the apoptosis pathway, playing a crucial role in programmed cell death. Dysregulation of caspase-9 has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
By detecting and quantifying caspase-9 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.With its user-friendly format and robust performance, the Mouse Casp9 ELISA Kit is an essential tool for scientists studying apoptosis, cell death, and related pathways in mouse models. Trust in this kit for accurate and precise measurements of caspase-9 levels in your research samples.
Product Name:
Mouse CASP9 (Caspase 9) ELISA Kit
SKU:
MOES00814
Target:
Mouse CASP9 (Caspase 9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CASP9. You can calculate the concentration of Mouse CASP9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
92-106
89-105
Average (%)
103
99
96
1:4
Range (%)
89-99
86-97
84-97
Average (%)
94
91
90
1:8
Range (%)
89-101
82-95
87-103
Average (%)
95
89
94
1:16
Range (%)
89-103
84-96
87-102
Average (%)
96
89
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.28
4.04
0.57
1.23
4.02
Standard deviation
0.03
0.05
0.15
0.04
0.07
0.13
C V (%)
5.56
3.91
3.71
7.02
5.69
3.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CASP9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CASP9 in samples. No significant cross-reactivity or interference between Mouse CASP9 and analogues was observed.
