The Mouse Casp8 (Caspase-8) ELISA Kit is specifically designed for the accurate detection of caspase-8 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Caspase-8 is a key protein involved in apoptosis, playing a critical role in regulating cell death and survival. Dysregulation of caspase-8 has been linked to various diseases, including cancer, autoimmune disorders, and neurodegenerative diseases, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
With its user-friendly format and detailed instructions, the Mouse Casp8 ELISA Kit is an essential tool for researchers investigating the role of caspase-8 in disease pathogenesis and therapeutic interventions.
Product Name:
Mouse CASP8 (Caspase 8) ELISA Kit
SKU:
MOES00675
Target:
Mouse CASP8 (Caspase 8)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CASP8. You can calculate the concentration of Mouse CASP8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-112
95-106
100-117
Average (%)
106
101
106
1:4
Range (%)
88-101
83-96
82-97
Average (%)
94
89
88
1:8
Range (%)
94-106
85-99
85-99
Average (%)
100
90
91
1:16
Range (%)
90-105
83-95
87-102
Average (%)
96
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.36
4.58
0.52
1.33
4.37
Standard deviation
0.03
0.07
0.24
0.03
0.07
0.18
C V (%)
5.56
5.15
5.24
5.77
5.26
4.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CASP8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CASP8 in samples. No significant cross-reactivity or interference between Mouse CASP8 and analogues was observed.
