The Mouse Casp3 (Caspase-3) ELISA Kit is specially designed for the accurate measurement of caspase-3 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring reliable and reproducible results for a wide range of research applications.Caspase-3 is a key enzyme involved in the process of apoptosis, playing a crucial role in programmed cell death. Dysregulation of caspase-3 activity has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
Therefore, the measurement of caspase-3 levels is essential for understanding the underlying mechanisms of these diseases and developing targeted therapies.The Mouse Casp3 ELISA Kit from Assay Genie provides researchers with a powerful tool for studying apoptosis and investigating the role of caspase-3 in disease progression. With its high sensitivity and specificity, this kit offers accurate and dependable results, making it a valuable addition to any laboratory conducting research in the field of cell biology and molecular medicine.
Product Name:
Mouse CASP3 (Caspase 3) ELISA Kit
SKU:
MOES00811
Target:
Mouse CASP3 (Caspase 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CASP3. You can calculate the concentration of Mouse CASP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
97-113
90-104
Average (%)
95
104
97
1:4
Range (%)
88-101
80-91
82-95
Average (%)
95
85
89
1:8
Range (%)
91-106
87-98
85-97
Average (%)
98
92
91
1:16
Range (%)
87-100
81-94
84-96
Average (%)
92
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.29
4.47
0.5
1.4
4.2
Standard deviation
0.03
0.06
0.19
0.03
0.06
0.23
C V (%)
5.66
4.65
4.25
6.0
4.29
5.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CASP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CASP3 in samples. No significant cross-reactivity or interference between Mouse CASP3 and analogues was observed.
