The Mouse Carbonic Anhydrase IX (CA9) ELISA Kit is specifically designed for precise and accurate measurement of CA9 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for various research studies.CA9 is a key enzyme that plays a crucial role in regulating pH balance and facilitating tumor cell survival and growth. Its expression is commonly elevated in various cancers, making it a valuable biomarker for cancer research and potential therapeutic interventions.
With its reliable performance and ease of use, the Mouse CA9 ELISA Kit is an essential tool for researchers studying tumor biology, cancer progression, and potential therapeutic targets in mouse models. Get accurate and reproducible results with this high-quality ELISA kit from Assay Genie.
Product Name:
Mouse Carbonic Anhydrase IX (CA9) ELISA Kit
Product Code:
MODL00190
Size:
96 Assays
Target:
CA9
Synonyms:
CA-IX, CAIX, MN, pMW1, P54/58N, Carbonic Dehydratase, Renal cell carcinoma-associated antigen G250, RCC-Associated Protein G250, Carbonate dehydratase IX
Detection Method:
Sandwich
Range:
1.56-100pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
