The Mouse CALP (Calprotectin) ELISA Kit is a powerful tool for detecting calprotectin levels in mouse biological samples, including serum, plasma, and tissue lysates. This kit boasts high sensitivity and specificity, providing researchers with reliable and consistent results for a variety of experimental applications.Calprotectin, a member of the S100 protein family, is an important biomarker of inflammation and is involved in various immune responses. Elevated levels of calprotectin have been associated with inflammatory bowel diseases, rheumatoid arthritis, and other inflammatory conditions, making it a valuable target for research and diagnostic purposes.
With the Mouse CALP ELISA Kit, researchers can accurately measure calprotectin levels in their samples, providing valuable insights into the inflammatory processes at play in their experimental models. This kit is an essential tool for studying the role of calprotectin in immune responses and inflammatory diseases, offering a reliable and efficient way to assess calprotectin levels in mouse samples.
Product Name:
Mouse CALP (Calprotectin) ELISA kit
SKU:
MOES01549
Target:
Mouse CALP (Calprotectin) ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CALP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CALP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CALP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CALP. You can calculate the concentration of Mouse CALP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
99-115
96-113
Average (%)
93
105
104
1:4
Range (%)
92-103
80-93
86-99
Average (%)
98
86
92
1:8
Range (%)
92-106
85-97
86-101
Average (%)
97
91
93
1:16
Range (%)
91-104
83-94
86-98
Average (%)
97
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.33
21.34
99.35
10.83
21.63
91.01
Standard deviation
0.57
0.93
4.1
0.74
0.98
4.51
C V (%)
5.52
4.36
4.13
6.83
4.53
4.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CALP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CALP in samples. No significant cross-reactivity or interference between Mouse CALP and analogues was observed.
